Trilostane

Trilostane structural formula

CAS Number13647-35-3
Molecular FormulaC20H27NO3
Molecular Weight329.441
InChI KeyKVJXBPDAXMEYOA-CXANFOAXSA-N
LogP2.80
Synonyms
  • Trilostane
  • (4alpha,5alpha,17beta)-3,17-Dihydroxy-4,5-epoxyandrost-2-ene-2-carbonitrile
  • Androst-2-ene-2-carbonitrile, 4,5-epoxy-3,17-dihydroxy-, (4alpha,5alpha,17beta)-
  • 13647-35-3
  • Androstane-2-carbonitrile, 4,5-epoxy-17-hydroxy-3-oxo-, (2-alpha,4-alpha,5-alpha,17-beta)-
  • EINECS 237-133-0
  • 4-alpha,5-Epoxy-3,17-dihydroxy-5-alpha-androst-2-ene-2-carbonitrile
  • (2-alpha,4-alpha,5-alpha,17-beta)-4,5-Epoxy-17-hydroxy-3-oxoandrostane-2-carbonitrile
  • 4-alpha-5-Epoxy-17-beta-hydroxy-3-oxo-5-alpha-androstane-2-alpha-carbonitrile
  • Modrenal
  • Modrastane
  • Trilostano
  • Trilostanum
  • UNII-L0FPV48Q5R
  • Vetoryl

Applications:

HPLC Method for Analysis of Trilostane

September 28, 2018

HPLC Method for Trilostane on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Trilostane 

Trilostane, sold most commonly under the brand name “Vetoryl,” is a medication used to treat Cushing’s syndrome in dogs. Previously, it had been approved for human use, but was withdrawn in 1990s. Trilostane is a steroidogenesis inhibitor, not an aromatase inhibitor, may still inhibit estrogen synthesis by inhibiting androgen synthesis.

Trilostane can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a disodium phosphate. Detection is performed using UV.

ColumnNewcrom R1, 4.6 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 20/80%
BufferNa2HPO4 pH 6.5 – 10 mM
Flow Rate1.0 ml/min
DetectionUV, 252 nm

Class of Compounds
Drug,  Hydrophobic, Ionizable, Zwitterionic, Steroidogenesis inhibitor
Analyzing CompoundsTrilostane

Application Column

Newcrom R1

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Trilostane
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.