Selenite

CAS Number14124-67-5
Molecular FormulaO3Se-2
Molecular Weight126.97
InChI KeyMCAHWIHFGHIESP-UHFFFAOYSA-L
Synonyms
  • selenite
  • Selenite ion
  • 14124-67-5
  • KXO0259XJ1
  • DTXSID9074517
  • Selenite 8147
  • RefChem:56006
  • DTXCID8034586
  • UNII-KXO0259XJ1
  • Selenit
  • trioxoselenate(IV)
  • trioxoselenate(2-)
  • trioxidoselenate(2-)
  • SELENITE ANION
  • Selenium (IV) trioxide
  • Monohydrated selenuim dioxide
  • SeO3(2-)
  • CHEBI:18212
  • [SeO3](2-)
  • C05684
  • Q27102911

Applications:

HPLC Method for Separation of Chloride, Selenite, Selenate, and Phosphate on Chromni Column

October 5, 2026

HPLC Method for Analysis of Selenite, Selenate, Chloride, Sodium, Phosphate on Chromni Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Selenite, Selenate, Chloride, Sodium, Phosphate.

Chloride is typically used to refer to a compound or molecule that contains a chlorine anion: Cl–. It is an electrolyte that is essential for bodily functions including blood pH, fluid balance, cellular functions, and more. Imbalance of chloride can indicate underlying health problems.

Sodium, which  has a chemical symbol of Na, is a soft alkali metal that is highly reactive.  It is found in abundance in everyday materials like table salt, sea water, and even the Earth’s crust. It is crucial  for the body’s function and fluid balance.

Selenite, the anion, not the crystal, has the chemical formula SeO32-. It’s an oxyanion of selenium.

Phosphate, PO43-, is a compound that plays an important part in biological energy transfer. It contains phosphorus, which is also a key component in bones and teeth. It is also a buffer, which helps maintain pH levels in a body.

Selenate is an ion of selenium with the chemical formula SeO32. It is a good oxidizer, easily reduced to selenite and selenium.

Selenite, Selenate, Chloride, Sodium, Phosphate can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using ELSD.

Condition

ColumnChromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated
Mobile PhaseMeCN/H2O – 80/20
BufferAmFM pH 3.0 – 10 mM
Flow Rate1.0 ml/min
DetectionELSD, the nebulizer and evaporator temperatures
50°C with a gas flow rate of 1.6 Standard Liters per Minute (SLM)

Description

Class of CompoundsIons
Analyzing CompoundsSelenite, Selenate, Chloride, Sodium, Phosphate

Application Column

Chromni

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated

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Application Analytes:
Chloride
Phosphate
Selenate
Selenite
Sodium

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.