Protoheme

CAS Number104244-10-2
Molecular FormulaC34H32FeN4O4
Molecular Weight616.5
InChI KeyKABFMIBPWCXCRK-UHFFFAOYSA-L
Synonyms
  • Ferroprotoporphyrin
  • Haem
  • Heme
  • Heme b
  • Protoheme
  • Protoheme IX

Applications:

HPLC Method for Analysis of Heme B in Blood Samples on Newcrom R1 Column

August 7, 2024

High Performance Liquid Chromatography (HPLC) Method for Analysis of Protoheme on Newcrom R1 by SIELC Technologies

Separation type: Liquid Chromatography Reverse -phase SIELC Technologies

HPLC Method for Analysis of Heme B in Blood Samples on Newcrom R1 Column

Heme B, also known as protoheme IX, is a specific type of heme and falls under the class of porphyrin compounds. It is an iron-containing compound that is vital for various biological functions, primarily as a component of hemoglobin and myoglobin, which are essential for oxygen transport and storage in the body. Additionally, heme B is found in certain enzymes such as cytochromes, where it plays a key role in electron transport and redox reactions.

Protoheme can be retained, separated and analyzed using a Newcrom R1 reverse-phase stationary phase column. The analysis employs a gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and formic acid as a buffer. This method allows for detection using UV 400 nm.

You can find detailed UV spectra of Protoheme and information about its various lambda maxima by visiting the following link.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Protoheme on Newcrom R1 by SIELC Technologies

Condition

ColumnNewcrom R1, 3.2 x 100 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN- 40-80%, 10 min
BufferFormic Acid – 1%
Flow Rate0.5 ml/min
DetectionUV 400 nm, ESI+ SIM 616

Description

Class of CompoundsPorphyrins
Analyzing CompoundsProtoheme

Application Column

Newcrom R1

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Protoheme

Application Detection:
UV Detection
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.