High Performance Liquid Chromatography (HPLC) Method for Analysis of Protoheme on Newcrom R1 by SIELC Technologies
Separation type: Liquid Chromatography Reverse -phase SIELC Technologies
Heme B, also known as protoheme IX, is a specific type of heme and falls under the class of porphyrin compounds. It is an iron-containing compound that is vital for various biological functions, primarily as a component of hemoglobin and myoglobin, which are essential for oxygen transport and storage in the body. Additionally, heme B is found in certain enzymes such as cytochromes, where it plays a key role in electron transport and redox reactions.
Protoheme can be retained, separated and analyzed using a Newcrom R1 reverse-phase stationary phase column. The analysis employs a gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and formic acid as a buffer. This method allows for detection using UV 400 nm.
You can find detailed UV spectra of Protoheme and information about its various lambda maxima by visiting the following link.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Protoheme on Newcrom R1 by SIELC Technologies
Condition
| Column | Newcrom R1, 3.2 x 100 mm, 5 µm, 100 A |
| Mobile Phase | Gradient MeCN- 40-80%, 10 min |
| Buffer | Formic Acid – 1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 400 nm, ESI+ SIM 616 |
Description
| Class of Compounds | Porphyrins |
| Analyzing Compounds | Protoheme |
Application Column
Newcrom R1
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
LC MS Detection
