Nebivolol

CAS Number99200-09-6
Molecular FormulaC22H25F2NO4
Molecular Weight405.4
InChI KeyKOHIRBRYDXPAMZ-UHFFFAOYSA-N
LogP3
Synonyms
  • Nebivolol
  • 99200-09-6
  • 2,2'-Azanediylbis(1-(6-fluorochroman-2-yl)ethanol)
  • CHEBI:64019
  • Nebivololum [Latin]
  • 118457-14-0
  • 2,2'-iminobis[1-(6-fluoro-3,4-dihydro-2H-chromen-2-yl)ethanol]
  • R 67145
  • R 065824
  • 1-(6-fluoro-3,4-dihydro-2H-chromen-2-yl)-2-[[2-(6-fluoro-3,4-dihydro-2H-chromen-2-yl)-2-hydroxyethyl]amino]ethanol
  • (-)-Nebivolol; Levonebivolol; l-Nebivolol
  • 118457-16-2
  • R65,824
  • R-65824
  • (1R)-1-[(2S)-6-fluoro-3,4-dihydro-2H-chromen-2-yl]-2-[[(2R)-2-[(2S)-6-fluoro-3,4-dihydro-2H-chromen-2-yl]-2-hydroxyethyl]amino]ethanol
  • R 67138;d-Nebivolol;(+)-Nebivolol; (S,R,R,R)-Nebivolol
  • Narbivolol
  • Nebivolol-d4

Applications:

HPLC Chiral Separation of Nebivolol Enantiomers on Cyralis AM Column

August 25, 2026

HPLC Chiral Method for Analysis of Nebivolol Enantiomers on Cyralis AM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Nebivolol.

Nebivolol is a beta-blocker with the chemical formula C22H25F2NO4. It is an oral medication used to treat high blood pressure, but is not preferred to alternatives. Side effects of it include dizziness, fatigue, nausea, and headaches. You can find detailed UV spectra of Nebivolol and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Nebivolol enantiomers using SIELC’s Cyralis AM chiral column. Cyralis AM is an amylose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Nebivolol can be retained and analyzed using the Cyralis AM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, Ethanol, and DEA. Detection is performed using UV.

Condition

ColumnCyralis AM, 4.6 x 100 mm, 5 µm, 100 A, normal phase
Mobile PhaseHexane/Ethanol/DEA
Flow Rate1.0 mL/min
DetectionUV 280 nm
Resolution2.06

Description

Class of Compoundsbeta-blockers
Analyzing CompoundsNebivolol

Application Column

Cyralis AM

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: normal phase

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Application Analytes:
Nebivolol
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

UV-Vis Spectrum of Nebivolol

July 15, 2024

UV-Vis Spectrum of Nebivolol. Absorption Maxima: 194 nm, 281 nm.

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.

Application Analytes:
Nebivolol
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Nebivolol Analysis in Bystolic Tablet Dosage Form on Primesep SB Column

September 8, 2021

HPLC Method for Nebivolol, Bystolic on Primesep SB by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Nebivolol, Bystolic.

Nebivolol is a beta-blocker with the chemical formula C22H25F2NO4. It is an oral medication used to treat high blood pressure, but is not preferred to alternatives. Side effects of it include dizziness, fatigue, nausea, and headaches. You can find detailed UV spectra of Nebivolol, Bystolic and information about its various lambda maxima by visiting the following link.

Nebivolol can be retained in HPLC on Primesep SB reverse-phase (RP) mixed-mode column with embedded strong basic ion-pairing groups, using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. UV detection at 285 nm.

Condition
Column (Column variation not found)
Mobile Phase MeCN/H2O – 25/75%
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV 285 nm

Description
Class of Compounds
 Drug
Analyzing Compounds Nebivolol, Bystolic

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Bystolic
Nebivolol
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Bystolic (Nebivolol) on Primesep 200

May 6, 2016

Bystolic

Application Column

Primesep 200

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Bystolic
Nebivolol
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.