HPLC Method for Analysis of Metoprolol on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Metoprolol.

Metoprolol is a medication with the chemical formula C₁₅H₂₅NO₃. It is primarily used to treat angina, high blood pressure, abnormally fast heart rate, and to prevent heart problems after a myocardial infraction. It works through blocking β1-adrenergic receptors, which increase heartrate.

You can find detailed UV spectra of Metoprolol and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Metoprolol enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Metoprolol can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, Ethanol, and DEA. Detection is performed using UV.

Condition

Description

Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Metoprolol check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

HPLC Method for Metoprolol on Primesep 200 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Metoprolol 

Metoprolol is a medication with the chemical formula C₁₅H₂₅NO₃. It is primarily used to treat angina, high blood pressure, abnormally fast heart rate, and to prevent heart problems after a myocardial infraction. It works through blocking β1-adrenergic receptors, which increase heartrate.

Metoprolol can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.

HPLC Method for Metoprolol on Primesep 200 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Metoprolol 

Metoprolol is a beta-blocker that is used to treat high blood pressure, angina, and heart failure by blocking beta-1 adrenergic receptors. At it’s most effective, it can lower the risk of death after a heart attack.

Metoprolol can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.