HPLC Method for Analysis of Metoprolol on Primesep 200 Column on Alltesta™

HPLC Method for Metoprolol on Primesep 200 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Metoprolol 

Metoprolol is a medication with the chemical formula C15H25NO3. It is primarily used to treat angina, high blood pressure, abnormally fast heart rate, and to prevent heart problems after a myocardial infraction. It works through blocking β1-adrenergic receptors, which increase heartrate.

Metoprolol can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.

ColumnPrimesep 200, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 50%
BufferPhosphoric Acid
Flow Rate1.0 mL/min
DetectionUV 275 nm
Class of CompoundsDrug
Analyzing CompoundsMetoprolol

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Metoprolol
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.