| CAS Number | 51384-51-1 |
|---|---|
| Molecular Formula | C15H25NO3 |
| Molecular Weight | 267.369 |
| InChI Key | IUBSYMUCCVWXPE-UHFFFAOYSA-N |
| LogP | 1.88 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Metoprolol
February 17, 2026
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Metoprolol check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Analysis of Metoprolol on Primesep 200 Column on Alltesta™
December 1, 2025
HPLC Method for Metoprolol on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Metoprolol
Metoprolol is a medication with the chemical formula C15H25NO3. It is primarily used to treat angina, high blood pressure, abnormally fast heart rate, and to prevent heart problems after a myocardial infraction. It works through blocking β1-adrenergic receptors, which increase heartrate.
Metoprolol can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
| Column | Primesep 200, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 50% |
| Buffer | Phosphoric Acid |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Metoprolol |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
HPLC Method for Analysis of Metoprolol in Tablets on Primesep 200 Column
June 29, 2018
HPLC Method for Metoprolol on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Metoprolol
Metoprolol is a beta-blocker that is used to treat high blood pressure, angina, and heart failure by blocking beta-1 adrenergic receptors. At it’s most effective, it can lower the risk of death after a heart attack.
Metoprolol can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
| Column | Primesep 200, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H3PO4 – 0.5 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 223 nm |
| Class of Compounds |
Drug, Beta-blocker, Hydrophobic, Basic |
| Analyzing Compounds | Metoprolol |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
