| CAS Number | 66-27-3 |
|---|---|
| Molecular Formula | C2H6O3S |
| Molecular Weight | 110.130 |
| InChI Key | MBABOKRGFJTBAE-UHFFFAOYSA-N |
| LogP | -0.504 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Methyl Methanesulfonate
January 30, 2026
If you are looking for optimized HPLC method to analyze Methyl methanesulfonate check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
RI HPLC Method for Analysis of Methyl methanesulfonate (MMS) on Primesep N Column
February 3, 2025
HPLC Method for Methyl methanesulfonate on Primesep N by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Methyl methanesulfonate
Methyl methanesulfonate (MMS) is an organic compound with the molecular formula C₂H₆O₄S. It is a potent alkylating agent known for its ability to transfer methyl groups to DNA, proteins, and other cellular components. Due to its strong mutagenic and cytotoxic properties, MMS is widely used in scientific research to study DNA damage, repair mechanisms, and genotoxicity.
Methyl methanesulfonate can be retained and analyzed using the Primesep N stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting solely of isopropyl alcohol (IPA). Detection is performed using a Refractive Index (RI) detector.
| Column | Primesep N, 4.6 x 250 mm, 3 µm, 100 A, dual ended |
| Mobile Phase | IPA – 100% |
| Buffer | None |
| Flow Rate | 0.4 ml/min |
| Detection | RI |
| LOD | 12.9 ppm |
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
| Class of Compounds | Alkylating Agents |
| Analyzing Compounds | Methyl methanesulfonate |
Application Column
Primesep N
Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended
Separation of Methyl methanesulfonate on Newcrom R1 HPLC column
February 19, 2018
Methyl methanesulfonate can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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