RI HPLC Method for Analysis of Methyl methanesulfonate (MMS) on Primesep N Column

HPLC Method for Methyl methanesulfonate on Primesep N by SIELC Technologies

HPLC Method for Analysis of Methyl methanesulfonate on Primesep N Column by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Methyl methanesulfonate

Methyl methanesulfonate (MMS) is an organic compound with the molecular formula C₂H₆O₄S. It is a potent alkylating agent known for its ability to transfer methyl groups to DNA, proteins, and other cellular components. Due to its strong mutagenic and cytotoxic properties, MMS is widely used in scientific research to study DNA damage, repair mechanisms, and genotoxicity.

Methyl methanesulfonate can be retained and analyzed using the Primesep N stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting solely of isopropyl alcohol (IPA). Detection is performed using a Refractive Index (RI) detector.

ColumnPrimesep N, 4.6 x 250 mm, 3 µm, 100 A
Mobile PhaseIPA – 100%
BufferNone
Flow Rate0.4 ml/min
DetectionRI
LOD12.9 ppm

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsAlkylating Agents
Analyzing CompoundsMethyl methanesulfonate

Application Column

Primesep N

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 3 µm
Pore Size: 100 A

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Application Analytes:
Methyl methanesulfonate
Application Detection:
RI Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.