| CAS Number | 61-68-7 |
|---|---|
| Molecular Formula | C15H15NO2 |
| Molecular Weight | 241.290 |
| InChI Key | HYYBABOKPJLUIN-UHFFFAOYSA-N |
| LogP | 5.12 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Mefenamic acid on Newcrom BH Column
September 28, 2026
HPLC Method for Analysis of Mefenamic acid on Newcrom BH Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Mefenamic acid.
Mefenamic Acid is a nonsteroidal anti-inflammatory drug and an anthranilic acid derivative, also known as fenamate, with the formula C15H15NO2. It is used primarily to treat pain and inflammation in rheumatoid arthritis and osteoarthritis, postoperative pain, menstrual pain, menorrhagia, and more. It works similarly to other anthranilic acid derivatives by inhibiting isoforms of the enzyme cyclooxygenase, which prevents the formation of prostaglandins. You can find detailed UV spectra of Mefenamic acid and information about its various lambda maxima by visiting the following link.
Mefenamic acid can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) and ethanol. Detection is performed using UV.
Condition
| Column | Newcrom BH, 3.2 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 45/55% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 192 nm |
| LOD* | 1 ppb |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Mefenamic acid |
Application Column
Newcrom BH
Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Uv-Vis Spectrum of Mefenamic Acid
September 28, 2026
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Mefenamic acid check our HPLC Applications library
UV/Vis.: λmax: 192, 220 nm, 280 nm, 352 nm
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. This compound was diluted in a mixture of water and acetonitrile. The method was done with a mixture of water and acetonitrile.
HPLC Method for Analysis of Mefenamic Acid on Chromni Column
September 28, 2026
HPLC Method for Analysis of Mefenamic acid on Chromni Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Mefenamic acid.
Mefenamic Acid is a nonsteroidal anti-inflammatory drug and an anthranilic acid derivative, also known as fenamate, with the formula C15H15NO2. It is used primarily to treat pain and inflammation in rheumatoid arthritis and osteoarthritis, postoperative pain, menstrual pain, menorrhagia, and more. It works similarlyto other anthranilic acid derivatives by inhibiting isoforms of the enzyme cyclooxygenase, which prevents the formation of prostaglandins.
Mefenamic acid can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.
Condition
| Column | Chromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated |
| Mobile Phase | MeCN/H2O |
| Buffer | AmAc pH 7.0 – 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 290 nm |
| LOD* | 16.6 ppb |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Mefenamic acid |
Application Column
Chromni
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated
Separation of Mefenamic acid on Newcrom R1 HPLC column
February 16, 2018
Mefenamic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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