HPLC Method for Analysis of Mefenamic Acid on Chromni Column

HPLC Method for Analysis of Mefenamic acid on Chromni Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Mefenamic acid.

Mefenamic Acid is a nonsteroidal anti-inflammatory drug and an anthranilic acid derivative, also known as fenamate, with the formula C15H15NO2. It is used primarily to treat pain and inflammation in rheumatoid arthritis and osteoarthritis, postoperative pain, menstrual pain, menorrhagia, and more. It works similarlyto other anthranilic acid derivatives by inhibiting isoforms of the enzyme cyclooxygenase, which prevents the formation of prostaglandins.

Mefenamic acid can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.

Condition

ColumnChromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated
Mobile PhaseMeCN/H2O
BufferAmAc pH 7.0 – 10 mM
Flow Rate1.0 ml/min
DetectionUV 290 nm
LOD*16.6 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsDrug
Analyzing CompoundsMefenamic acid

Application Column

Chromni

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated

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Application Analytes:
Mefenamic acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.