HPLC Method for Analysis of Mefenamic acid on Newcrom BH Column

HPLC Method for Analysis of Mefenamic acid on Newcrom BH Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Mefenamic acid.

Mefenamic Acid is a nonsteroidal anti-inflammatory drug and an anthranilic acid derivative, also known as fenamate, with the formula C15H15NO2. It is used primarily to treat pain and inflammation in rheumatoid arthritis and osteoarthritis, postoperative pain, menstrual pain, menorrhagia, and more. It works similarly to other anthranilic acid derivatives by inhibiting isoforms of the enzyme cyclooxygenase, which prevents the formation of prostaglandins. You can find detailed UV spectra of Mefenamic acid and information about its various lambda maxima by visiting the following link.

Mefenamic acid can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) and ethanol. Detection is performed using UV.

Condition

ColumnNewcrom BH, 3.2 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 45/55%
BufferH2SO4 – 0.1%
Flow Rate0.5 ml/min
DetectionUV 192 nm
LOD*1 ppb
LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsDrug
Analyzing CompoundsMefenamic acid

Application Column

Newcrom BH

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Mefenamic acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.