Levofloxacin

Levofloxacin structural formula

CAS Number100986-85-4
Molecular FormulaC18H20FN3O4
Molecular Weight361.373
InChI KeyGSDSWSVVBLHKDQ-JTQLQIEISA-N
LogP-0.000191
Synonyms
  • Levofloxacin
  • (3S)-9-Fluoro-3-methyl-10-(4-methylpiperazin-1-yl)-7-oxo-2,3-dihydro-7H-[1,4]oxazino[2,3,4-ij]quinoline-6-carboxylic acid
  • 7H-1,4-Oxazino[2,3,4-ij]quinoline-6-carboxylic acid, 9-fluoro-2,3-dihydro-3-methyl-10-(4-methyl-1-piperazinyl)-7-oxo-, (3S)-
  • 100986-85-4
  • 7H-Pyrido[1,2,3-de]-1,4-benzoxazine-6-carboxylic acid, 9-fluoro-2,3-dihydro-3-methyl-10-(4-methyl-1-piperazinyl)-7-oxo-, (3S)-
  • (S)-9-Fluoro-2,3-dihydro-3-methyl-10-(4-methyl-1-piperazinyl)-7-oxo-7H-pyrido(1,2,3-de)-1,4-benzoxazine-6-carboxylic acid
  • (-)-Ofloxacin
  • (S)-Ofloxacin
  • RWJ 25213-097
  • Ofloxacin S-(-)-form
  • (S)-(-)-Ofloxacin
  • Elequine
  • Levaquin in dextrose 5% in plastic container
  • Levofloxacine
  • Levofloxacino
  • Levofloxacinum
  • Oftaquix
  • Tavanic
  • UNII-RIX4E89Y14
  • Unibiotic
  • (2S)-7-fluoro-2-methyl-6-(4-methylpiperazin-1-yl)-10-oxo-4-oxa-1-azatricyclo[7.3.1.05,13]trideca-5(13),6,8,11-tetraene-11-carboxylic acid
  • Floxin
  • L-Ofloxacin
  • Levofloxacin hydrate
  • Ofloxacin
  • Quixin

Applications:

HPLC Method for Analysis of Levofloxacin

August 14, 2020

HPLC Method for Levofloxacin on Primesep 200 by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Levofloxacin.

Levofloxacin is a fluoroquinolone antibiotic with the chemical formula C18H20FN3O4. Due to its potential for irreversible side effects, it is only used in cases for treatment when there is no other option left. It works through inhibiting two bacterial enzymes, DNA gyrase and topoisomerase IV, which prevents bacteria from multiplying.

Levofloxacin can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

Condition 1
Column Primesep 200, 4.6×100 mm, 5 µm, 100A
Mobile Phase MeCN Gradient
Buffer H2SO4 Gradient
Flow Rate 1.0 ml/min
Detection UV 275 nm

Condition 2
Column Primesep SB, 4.6×100 mm, 5 µm, 100A
Mobile Phase MeCN Gradient
Buffer AmFm pH 3.0 – 30 mM
Flow Rate  1 ml/min
Detection UV 275 nm

 

Description
Class of Compounds Drugs, Antibiotics
Analyzing Compounds Levofloxacin

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Levofloxacin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Levofloxacin on Newcrom R1 HPLC column

February 16, 2018
Separation of Levofloxacin on Newcrom C18 HPLC column

Levofloxacin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Levofloxacin
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.