| CAS Number | 100986-85-4 |
|---|---|
| Molecular Formula | C18H20FN3O4 |
| Molecular Weight | 361.373 |
| InChI Key | GSDSWSVVBLHKDQ-JTQLQIEISA-N |
| LogP | -0.000191 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Levofloxacin
August 14, 2020
| Column | Primesep 200, 4.6×100 mm, 5 µm, 100A |
| Mobile Phase | MeCN Gradient |
| Buffer | H2SO4 Gradient |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm |
| Column | Primesep SB, 4.6*100 mm, 5 µm, 100A |
| Mobile Phase | MeCN Gradient |
| Buffer | AmFm pH 3.0 – 30 mM |
| Flow Rate | 1 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Drugs, Antibiotics |
| Analyzing Compounds | Levofloxacin |
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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Primesep SB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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Separation of Levofloxacin on Newcrom R1 HPLC column
February 16, 2018
Levofloxacin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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