HPLC Method for Analysis of Levofloxacin

HPLC Method for Levofloxacin on Primesep 200 by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Levofloxacin.

Levofloxacin is a fluoroquinolone antibiotic with the chemical formula C18H20FN3O4. Due to its potential for irreversible side effects, it is only used in cases for treatment when there is no other option left. It works through inhibiting two bacterial enzymes, DNA gyrase and topoisomerase IV, which prevents bacteria from multiplying.

Levofloxacin can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

Condition 1
Column Primesep 200, 4.6×100 mm, 5 µm, 100A
Mobile Phase MeCN Gradient
Buffer H2SO4 Gradient
Flow Rate 1.0 ml/min
Detection UV 275 nm

Condition 2
Column Primesep SB, 4.6×100 mm, 5 µm, 100A
Mobile Phase MeCN Gradient
Buffer AmFm pH 3.0 – 30 mM
Flow Rate  1 ml/min
Detection UV 275 nm

 

Description
Class of Compounds Drugs, Antibiotics
Analyzing Compounds Levofloxacin

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Levofloxacin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.