LC MS-HPLC Method for Analysis of Defactinib on Primesep B Column

HPLC Method for Defactinib on Primesep B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Defactinib

Defactinib is a focal adhesion kinase (FAK) inhibitor with the chemical formula C20H21F3N8O3S. It was developed by Pfizer as potential treatment for solid tumors. You can find detailed UV spectra of Defactinib and information about its various lambda maxima by visiting the following link.

Defactinib can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

ColumnPrimesep B, 2.1 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 40/60 %
BufferAmFm pH 3.0 – 10 mM 
Flow Rate0.2 ml/min
DetectionESI+ SIM 511+, ([M+H]1⁺)  UV 277, 305 nm
Class of Compounds
 Inhibitor
Analyzing CompoundsDefactinib

Application Column

Primesep B

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Defactinib
Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.