| CAS Number | 1073154-85-4 |
|---|---|
| Molecular Formula | C20H21F3N8O3S |
| Molecular Weight | 510.5 |
| InChI Key | FWLMVFUGMHIOAA-UHFFFAOYSA-N |
| LogP | 2.4 |
| Synonyms |
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Applications:
LC MS-HPLC Method for Analysis of Defactinib on Primesep B Column
March 16, 2026
HPLC Method for Defactinib on Primesep B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Defactinib
Defactinib is a focal adhesion kinase (FAK) inhibitor with the chemical formula C20H21F3N8O3S. It was developed by Pfizer as potential treatment for solid tumors. You can find detailed UV spectra of Defactinib and information about its various lambda maxima by visiting the following link.
Defactinib can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium format as a buffer. Detection is performed using ESI+Sim and UV.
| Column | Primesep B, 2.1 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 40/60 % |
| Buffer | AmFm pH 3.0 – 20 mM |
| Flow Rate | 0.2 ml/min |
| Detection | ESI+ SIM 511+, ([M+H]1⁺) UV 277, 305 nm |
| LOD* | ESI-SIM 4 ppb |
| Class of Compounds | Inhibitor |
| Analyzing Compounds | Defactinib |
Application Column
Primesep B
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
HPLC Method for Analysis of Defactinib on Primesep B Column
March 16, 2026
HPLC Method for Defactinib on Primesep B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Defactinib
Defactinib is a focal adhesion kinase (FAK) inhibitor with the chemical formula C20H21F3N8O3S. It was developed by Pfizer as potential treatment for solid tumors. You can find detailed UV spectra of Defactinib and information about its various lambda maxima by visiting the following link.
Defactinib can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN/H2O – 30/70% – 70/30%, 10 min |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/m |
| Detection | UV 200 nm |
| Class of Compounds | Inhibitor |
| Analyzing Compounds | Defactinib |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
UV-Vis Spectrum of Defactinib
July 16, 2025
If you are looking for optimized HPLC method to analyze Defactinib check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
