LC MS-HPLC Method for Analysis of Aceclidine on Primesep 200 Column

HPLC Method for Aceclidine on Primesep 200 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Aceclidine

Aceclidine Drug class: Muscarinic cholinergic agonist

Mechanism of action: Stimulates muscarinic receptors, leading to smooth muscle contraction and glandular secretion.

Historical use: Studied for glaucoma, gastrointestinal motility, and other parasympathetic-related effects

2025 FDA Approval:

  • Approved as VIZZ (aceclidine 1.44% ophthalmic solution)
  • Indication: Treatment of presbyopia (age-related near vision loss)

You can find detailed UV spectra of Aceclidine and information about its various lambda maxima by visiting the following link.

Aceclidine can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Ammonium format as a buffer. Detection is performed using ESI+ SIM.

ColumnPrimesep 200, 2.1 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 40/60 %
BufferAmFm pH 3.0 – 60 mM 
Flow Rate0.2 ml/min
DetectionESI+ SIM 170+, ([M+H]1⁺)
Class of Compounds
 Drug
Analyzing CompoundsAceclidine

Application Column

Primesep 200

Column Diameter: 2.1 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Aceclidine
Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.