| CAS Number | 4618-18-2 |
|---|---|
| Molecular Formula | C12H22O11 |
| Molecular Weight | 342.30 |
| InChI Key | JCQLYHFGKNRPGE-FCVZTGTOSA-N |
| LogP | -4.3 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Lactulose
February 3, 2026
If you are looking for optimized HPLC method to analyze Lactulose check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Analysis of Sucralose, Mannitol, and Lactulose on Primesep S2 Column Using Mass Spectrometry
August 13, 2025
HPLC Method for Sucralose, Mannitol, Lactulose on Primesep S2 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sucralose, Mannitol, Lactulose
Sucralose is an synthetic organochlorine compound with the molecular formula C12H19Cl3O8.
Properties:
Appearance: Typically a white, odorless, crystalline powder.
Molecular weight: ~397.6 g/mol
Solubility: Soluble in water.
Uses: An artificial sweetener used to provide sweetness in food and beverages without the calories of sugar.
Mannitol is an organic compound with the molecular formula C6H14O6.
Properties:
Appearance: Typically a white, odorless, crystalline powder or granules.
Molecular weight: ~182.17 g/mol
Solubility: Soluble in water.
Uses: A versatile medication with various uses in healthcare, primarily as an osmotic diuretic.
Lactulose is an synthetic disaccharide with the molecular formula C12H22O11.
Properties:
Appearance: Typically a white, odorless crystalline powder with a sweet taste.
Molecular weight: ~342.30 g/mol
Solubility: Soluble in water.
Uses: Used for treating constipation and hepatic encephalopathy (HE).
Sucralose, Mannitol, Lactulose can be retained and analyzed using the Primesep S2 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and formic acid acid. Detection is performed using MS.
| Column | Primesep S2, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 85% |
| Buffer | Formic Acid – 0.2% |
| Flow Rate | 0.5 ml/min |
| Detection | MS ESI – |
Application Column
Primesep S2
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Mannitol
Sucralose
HPLC Method for Analysis of Sucralose, Mannitol, and Lactulose on Primesep S2 Column Using ELSD
August 13, 2025
HPLC Method for Sucralose, Mannitol, Lactulose on Primesep S2 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sucralose, Mannitol, Lactulose
Sucralose is an synthetic organochlorine compound with the molecular formula C12H19Cl3O8.
Properties:
Appearance: Typically a white, odorless, crystalline powder.
Molecular weight: ~397.6 g/mol
Solubility: Soluble in water.
Uses: An artificial sweetener used to provide sweetness in food and beverages without the calories of sugar.
Mannitol is an organic compound with the molecular formula C6H14O6.
Properties:
Appearance: Typically a white, odorless, crystalline powder or granules.
Molecular weight: ~182.17 g/mol
Solubility: Soluble in water.
Uses: A versatile medication with various uses in healthcare, primarily as an osmotic diuretic.
Lactulose is an synthetic disaccharide with the molecular formula C12H22O11.
Properties:
Appearance: Typically a white, odorless crystalline powder with a sweet taste.
Molecular weight: ~342.30 g/mol
Solubility: Soluble in water.
Uses: Used for treating constipation and hepatic encephalopathy (HE).
Sucralose, Mannitol, Lactulose can be retained and analyzed using the Primesep S2 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and formic acid acid. Detection is performed using ELSD.
| Column | Primesep S2, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 85% |
| Buffer | Formic Acid – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
Application Column
Primesep S2
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Mannitol
Sucralose
HPLC Method for Analysis of Lactulose on Primesep S2 Column
August 12, 2025
HPLC Method for Lactulose on Primesep S2 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Lactulose
Lactulose is an synthetic disaccharide with the molecular formula C12H22O11.
Properties:
Appearance: Typically a white, odorless crystalline powder with a sweet taste.
Molecular weight: ~342.30 g/mol
Solubility: Soluble in water.
Uses: Used for treating constipation and hepatic encephalopathy (HE).
Lactulose can be retained and analyzed using the Primesep S2 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and formic acid. Detection is performed using ELSD.
| Column | Primesep S2, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 80% |
| Buffer | Formic Acid – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| LOD | 7.643 ppm |
| Class of Compounds | Synthetic Disaccharide |
| Analyzing Compounds | Lactulose |
Application Column
Primesep S2
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
