HPLC Method for Dopamine, Epinephrine, Norepinephrine on Newcrom AH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Dopamine, Epinephrine, Norepinephrine.

Catecholamines are neurotransmitters that generate the ‘fight-or-flight’ responsein the body. The 3 main catecholamines are epinephrine (also known as adrenaline), norepinephrine, and dopamine, and they are produced by the adrenal glands.

Norepinephrine, also known as noradrenaline and noradrenalin, is a catecholamine with the chemical formula C₈H₁₁NO₃. It functions as a hormone, neurotransmitter, and neuromodulator responsible for improving alertness, focus, memory, and regulating mood. You can find detailed UV spectra of Norepinephrine and information about its various lambda maxima by visiting the following link.

Epinephrine, also known as adrenaline or adrenalin,  is a a hormone and medication with the chemical formula C₉H₁₃NO₃. As a hormone, it is involved in regulating  involuntary operations of nearly all internal organs. As a medication, it is used to treat allergic reaction anaphylaxis and cardiac arrest. When other treatments are not effective, it is occasionally used to treat asthma. You can find detailed UV spectra of Epinephrine and information about its various lambda maxima by visiting the following link.

Dopamine is a neurotransmitter with the chemical formula C₈H₁₁NO_2. It is released during pleasurable activities, reinforcing those behaviors, and motivating people to continue seeking them out. Dopamine influences the release of other hormones. A deficiency of it can influence movement and lead to conditions like Parkinson’s disease. You can find detailed UV spectra of Dopamine and information about its various lambda maxima by visiting the following link.

Dopamine, Epinephrine, Norepinephrine  can be detected in the low UV regime. Using a Newcrom AH mixed-mode column and a mobile phase consisting of almost entirely water with either a phosphoric (H3PO4) acid or ammonium formate (AmFm) buffer, the catecholamines can be retained, separated, and measured. This analysis method can be UV detected at 275 nm with high resolution. The latter mobile phase (utilizing AmFm) is compatible with Mass Spectrometry.