HPLC Method for Analysis of Benzonitrile, Toluene, Benzylamine on Primesep A by SIELC Technologies
Benzonitrile is an aromatic organic compound with the chemical formula C₆H₅CN. It is a colorless liquid with an almond-like odor. It is primarily used as a solvent and a chemical intermediate in the production of a variety of compounds, including pharmaceuticals, dyes, and perfumes. Industrially, it is used to synthesize benzoguanamine, which is a precursor to melamine resin.
Toluene is a colorless, water-insoluble liquid with the chemical formula C₇H₈. It’s smell associated with paint thinners. It is a mono-substituted benzene derivative, consisting of a CH₃ group attached to a phenyl group. It is an important organic solvent and is also used to produce benzene, and a number of consumer products including certain types of glue, paint thinners, and nail polish removers.
Benzylamine is a primary amine compound containing a benzyl group attached to the amino group. It has the chemical formula C₆H₅CH₂NH₂. It is a colorless liquid with a faint almond-like odor that is used as a chemical intermediate in the synthesis of a variety of substances, including pharmaceuticals, dyes, and polymers. You can find detailed UV spectra of Benzylamine and information about its various lambda maxima by visiting the following link.
These chemicals are widely used in organic synthesis. Please handle these substances with care, as they can have hazardous effects. Always refer to the safety data sheets for each chemical and adhere to recommended safety procedures.
Benzonitrile, Toluene, Benzylamine be separate and analyzed on a reverse-phase Primesep A column with a mobile phase consisting of water, Acetonitrile (MeCN) and Sulfuric acid as a buffer modifier. This analysis method can be UV detected at 210 nm.
LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Condition
| Column | Primesep A, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O -60/40% |
| Buffer | H2SO4 |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Peaks Retention Time | 2.9, 4.0, 10.2 min |
| Samples concentration (Benzonitrile, Toluene, Benzylamine) | 0.04, 0.05, 0.07 mg/ml |
| Injection volume | 3 µl |
| Sample diluent | MeCN/H2O -60/40% |
| LOD (Benzonitrile, Toluene, Benzylamine) | 27, 65, 103 ppb |
Description
| Class of Compounds | Benzene, Aromatics |
| Analyzing Compounds | Benzonitrile, Toluene, Benzylamine |
Application Column
Primesep A
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Benzylamine
Toluene
