HPLC Method for Analysis of Acetazolamide on Primesep 100 Column

HPLC Method for Analysis of Acetazolamide on Primesep NC by SIELC Technologies

HPLC Method for Analysis of Acetazolamide in on Primesep 100 Column

Acetazolamide is a chiral aromatic amine with the molecular formula C4H6N4O3S2. It is used primarily to treat glaucoma, epilepsy, acute mountain sickness, heart failure, and more. It works as a carbonic anhydrase inhibitor, which causes the accumulation of carbonic acid, which is found in a wide variety of tissue. It is on the World Health Organization’s List of Essential Medicines. You can find detailed UV spectra of [compounds] and information about its various lambda maxima by visiting the following link.

Acetazolamide can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of acetonitrile, water, and Ammonium Formate. Detection is performed using UV.

Condition

ColumnPrimesep 100, 4.6 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O -40/60%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 266 nm
LOD*20 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsDrug
Analyzing CompoundsAcetazolamide

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Acetazolamide
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.