HPLC Method for Analysis of Nicotinamide and Pyridoxine on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Nicotinamide, Vitamin B6 (Pyridoxine) on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Nicotinamide, Vitamin B6 (Pyridoxine)

Nicotinamide is a specific form of vitamin B3, an essential nutrient that is crucial for various bodily functions. Along with niacin (nicotinic acid), nicotinamide is one of the primary forms of vitamin B3 that the body uses. Both of these forms are converted into coenzymes NAD+ and NADP+, which play vital roles in energy production, DNA repair, and cellular communication. This conversion is essential for maintaining the body’s metabolic processes, highlighting the importance of nicotinamide as a form of vitamin B3.

Pyridoxine is a form of vitamin B6, which is essential for numerous biochemical processes in the body. Vitamin B6 exists in three natural forms—pyridoxine, pyridoxal, and pyridoxamine—all of which are converted into the active coenzyme pyridoxal 5′-phosphate (PLP). This active form is critical for amino acid metabolism, neurotransmitter synthesis, and hemoglobin production. As a form of vitamin B6, pyridoxine is key to maintaining proper neurological and immune function, ensuring that the body can perform a wide range of essential functions.

Nicotinamide, Vitamin B6 (Pyridoxine) can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 270 nm.

You can find detailed UV spectra of Nicotinamide, Vitamin B6 (Pyridoxine) and information about its various lambda maxima by visiting the following links: UV-Vis Spectrum of Nicotinamide, UV-Vis Spectrum of Pyridoxine.