HPLC Method for Determination of Micafungin on Newcrom R1 Column

HPLC Method for Micafungin on Newcrom R1 by SIELC Technologies

HPLC Method for Determination of Micafungin on Newcrom R1 Column      

High Performance Liquid Chromatography (HPLC) Method for Analysis of Micafungin

Micafungin is an anti-fungal medication with the chemical formula C56H71N9O23S.  It is used to treat infections including but not limited to candidemia, abscesses, and esophageal candidiasis. It works through inhibiting the production of beta-1,3-glucan. It is only accessible through perscription.

Micafungin can be retained and analyzed on a Newcrom R1 reverse-phase column using a simple isocratic analytical method with a mobile phase consisting of Acetonitrile (MeCN), water, and Sulfuric acid as the ionic modifier. This analysis method can be UV detected at 270 nm with high resolution and peak symmetry.

Condition
Column Newcrom R1, 2.1 x 100 mm, 3 µm, 100 A, dual ended
Mobile Phase MeCN – 45%
Buffer H2SO4 – 0.2%
Flow Rate 0.2 ml/min
Detection UV 270 nm
LOD 0.02 ppm

 

Description
Class of Compounds Drug
Analyzing Compounds Micafungin

Application Column

Newcrom R1

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Micafungin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.