Access the UV-Vis Spectrum SIELC Library

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For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase.  The compound was dissolved with acetonitrile. This analysis was done with the mobile phase made of 20% acetonitrile and 80% water.

HPLC Method for Micafungin on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Micafungin

Micafungin is an anti-fungal medication with the chemical formula C₅₆H₇₁N₉O₂₃S.  It is used to treat infections including but not limited to candidemia, abscesses, and esophageal candidiasis. It works through inhibiting the production of beta-1,3-glucan. It is only accessible through perscription.

Micafungin can be retained and analyzed on a Newcrom R1 reverse-phase column using a simple isocratic analytical method with a mobile phase consisting of Acetonitrile (MeCN), water, and Sulfuric acid as the ionic modifier. This analysis method can be UV detected at 270 nm with high resolution and peak symmetry.