HPLC Method for Analysis of Ergocalciferol, Cholecalciferol on Primesep SB by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ergocalciferol, Cholecalciferol
Ergocalciferol (Vitamin D2) and Cholecalciferol (Vitamin D3) are fat soluble vitamins that help your body absorb calcium and phosphorus. Your body can produce both when exposed to the sun, but you can also intake these essential vitamins through a daily multivitamin or a Vitamin D pill.
Vitamins D2 and D3 can be detected in the high UV regime. Using a Primesep SB reverse-phase column and a mobile phase consisting of water and acetonitrile with an acetic acid buffer, Vitamins D2 and D3 can be retained and separated. This analysis method can be UV detected at 265 nm.
| Column | Primesep SB, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 80/20% |
| Buffer | Acetic Acid – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 265 nm |
| Class of Compounds | Vitamin |
| Analyzing Compounds | Ergocalciferol (Vitamin D2), Cholecalciferol (Vitamin D3) |
Application Column
Primesep SB
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
CholecalciferolErgocalciferol
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
