HPLC Method For Analysis of Ergocalciferol (Vitamin D2) and Cholecalciferol (Vitamin D3) on Primesep SB Column
September 1, 2021
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ergocalciferol (Vitamin D2) and Cholecalciferol (Vitamin D3)
Ergocalciferol (Vitamin D2) and Cholecalciferol (Vitamin D3) are fat soluble vitamins that help your body absorb calcium and phosphorus. Your body can produce both when exposed to the sun, but you can also intake these essential vitamins through a daily multivitamin or a Vitamin D pill.
Vitamins D2 and D3 can be detected in the high UV regime. Using a Primesep SB reverse-phase column and a mobile phase consisting of water and acetonitrile with an acetic acid buffer, Vitamins D2 and D3 can be retained and separated. This analysis method can be UV detected at 265 nm.
|Primesep SB, 4.6×150 mm, 5 µm, 100A
|MeCN/H2O – 80/20%
|Acetic Acid – 0.1%
|UV 265 nm
|Class of Compounds
|Ergocalciferol (Vitamin D2), Cholecalciferol (Vitamin D3)
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.Select options
Separation of Ergocalciferol on Newcrom R1 HPLC column
February 16, 2018
Ergocalciferol can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.Select options