Ergocalciferol

Ergocalciferol structural formula

CAS Number50-14-6
Molecular FormulaC28H44O
Molecular Weight396.659
InChI KeyMECHNRXZTMCUDQ-RKHKHRCZSA-N
LogP8.83
Synonyms
  • Ergocalciferol
  • (3S,5Z,7E,22E)-9,10-Secoergosta-5,7,10,22-tetraen-3-ol
  • Cyclohexanol, 4-methylene-3-[(2E)-2-[(1R,3aS,7aR)-octahydro-7a-methyl-1-[(1R,2E,4R)-1,4,5-trimethyl-2-hexen-1-yl]-4H-inden-4-ylidene]ethylidene]-, (1S,3Z)-
  • 50-14-6
  • Calciferol
  • 9,10-Secoergosta-5,7,10(19),22-tetraen-3-ol, (3?,5Z,7E,22E)-
  • (+)-Vitamin D2
  • 9,10-Secoergosta-5,7,10(19),22-tetraen-3?-ol
  • Condocaps
  • Crystallina
  • Cyclohexanol, 4-methylene-3-[(2E)-2-[(1R,3aS,7aR)-octahydro-7a-methyl-1-[(1R,2E,4R)-1,4,5-trimethyl-2-hexen-1-yl]-4H-inden-4-ylidene]ethylidene]-, (1S,3Z)-
  • Cyclohexanol, 4-methylene-3-[2-[tetrahydro-7a-methyl-1-(1,4,5-trimethyl-2-hexenyl)-4(3aH)-indanylidene]ethylidene]-
  • D-Arthin
  • Davitamon D
  • Davitin
  • Dee-Osterol
  • Dee-Ron
  • Dee-Ronal
  • Deltalin
  • Deratol
  • Detalup
  • Diactol
  • Divit urto
  • Drisdol
  • D-Tracetten
  • Ercalciol
  • Ergorone
  • Fortodyl
  • Geltabs
  • Hi-Deratol
  • Metadee
  • Mulsiferol
  • Mykostin
  • NSC 62792
  • Oleovitamin D
  • Oleovitamin D2
  • Ostelin
  • Radiostol
  • Radsterin
  • Rodine C
  • Shock-ferol
  • Sterogyl
  • Uvesterol D
  • Viosterol
  • Vitavel-D
  • Activated ergosterol
  • Calciferolum
  • EINECS 200-014-9
  • Hyperkil
  • Irradiated ergosta-5,7,22-trien-3-beta-ol
  • Rodinec
  • (3-beta,5Z,7E,22E)-9,10-Secoergosta-5,7,10,(19),22-tetraen-3-ol
  • 9,10-Seco(5Z,7E,22E)-5,7,10(19),22-ergostatetraen-3-ol
  • 9,10-Secoergosta-5,7,10(19),22-tetraen-3-beta-ol
  • Sorex C.R.
  • Ergocalciferolo
  • Ergocalciferolum
  • UNII-VS041H42XC
  • Disdrol
  • (1S,3Z)-3-[(2E)-2-[(1R,3aS,7aR)-1-[(E,2R,5R)-5,6-dimethylhept-3-en-2-yl]-7a-methyl-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylidene-cyclohexan-1-ol
  • (1S,3Z)-3-[(2E)-2-[(1R,3aS,7aR)-1-[(E,2R,5R)-5,6-dimethylhept-3-en-2-yl]-7a-methyl-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylidenecyclohexan-1-ol
  • (1S,3Z)-3-[(2E)-2-[(1R,3aS,7aR)-7a-methyl-1-[(E,1R,4R)-1,4,5-trimethylhex-2-enyl]-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylene-1-cyclohexanol
  • (1S,3Z)-3-[(2E)-2-[(1R,3aS,7aR)-7a-methyl-1-[(E,1R,4R)-1,4,5-trimethylhex-2-enyl]-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylene-cyclohexan-1-ol
  • (1S,3Z)-3-{2-[(1R,3aS,4E,7aR)-1-[(2R,3E,5R)-5,6-dimethylhept-3-en-2-yl]-7a-methyl-octahydro-1H-inden-4-ylidene]ethylidene}-4-methylidenecyclohexan-1-ol
  • (3beta,5Z,7E,22E)-9,10-secoergosta-5,7,10(19),22-tetraen-3-ol
  • (5E,7E,22E)-9,10-Secoergosta-5,7,10,22-tetraen-3-ol
  • (5Z,7E,22E)-(3S)-9,10-seco-5,7,10(19),22-ergostatetraen-3-ol
  • (5Z,7E,22E)-(3S)-9,10-secoergosta-5,7,10(19),22-tetraen-3-ol
  • 22-Tetraen 3beta 9,10,Secoergosta-5,7,10(19)-ol
  • 4-Methylene-3-[2-[tetrahydro-7a-methyl-1-(1,4,5-trimethyl-2-hexenyl)-4(3aH)-indanylidene]ethylidene]-Cyclohexanol
  • 9,10-Secoergosta-5,7,10(19),22-tetraen-3b-ol
  • Buco-D
  • Calciferon 2
  • Condacaps
  • Condol
  • Crtron
  • Daral
  • Davitin
  • De-rat concentrate
  • Decaps
  • Dee-Roual
  • Doral
  • Ergocalciferol oil
  • Ergosterol activated
  • Ergosterol irradiated
  • Ertron
  • Infron
  • Irradiated ergosta-5,7,22-trien-3beta-ol
  • Mina D2
  • Novovitamin-D
  • Osteil
  • Radiostol
  • Radstein
  • Shock-ferol sterogyl
  • Synthetic Vitamin D
  • Uvesterol-D
  • Vio D
  • Vio-D
  • Viostdrol
  • Viosterol in Oil
  • Vitamina D2
  • activated ergosterol
  • beta-Ol
  • delta-Arthin
  • delta-Tracetten
  • vitamin D2
  • 1384584-60-4
  • 31316-19-5
  • 7489-18-1
  • 8017-28-5

Applications:

Uv-Vis Spectrum of Ergocalciferol

March 16, 2026

Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Ergocalciferol check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The compound was dissolved in methanol.

Application Analytes:
Ergocalciferol
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method For Analysis of Ergocalciferol (Vitamin D2) and Cholecalciferol (Vitamin D3) on Primesep SB Column

September 1, 2021

HPLC Method for Analysis of Ergocalciferol, Cholecalciferol on Primesep SB by SIELC Technologies

HPLC Method For Analysis of Vitamin D2 and D3 on Primesep SB Column_1401
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ergocalciferol, Cholecalciferol

Ergocalciferol (Vitamin D2) and Cholecalciferol (Vitamin D3) are fat soluble vitamins that help your body absorb calcium and phosphorus. Your body can produce both when exposed to the sun, but you can also intake these essential vitamins through a daily multivitamin or a Vitamin D pill.


Vitamins D2 and D3 can be detected in the high UV regime. Using a Primesep SB reverse-phase column and a mobile phase consisting of water and acetonitrile with an acetic acid buffer, Vitamins D2 and D3 can be retained and separated. This analysis method can be UV detected at 265 nm.

ColumnPrimesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 80/20%
BufferAcetic Acid – 0.1%
Flow Rate1.0 ml/min
DetectionUV 265 nm
Class of Compounds
Vitamin
Analyzing CompoundsErgocalciferol, Cholecalciferol

Application Column

Primesep SB

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Cholecalciferol
Ergocalciferol
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Ergocalciferol on Newcrom R1 HPLC column

February 16, 2018
Separation of Ergocalciferol on Newcrom C18 HPLC column

Ergocalciferol can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Ergocalciferol
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.