HPLC Method For Analysis of Ergocalciferol (Vitamin D2) and Cholecalciferol (Vitamin D3) on Primesep SB Column

HPLC Method for Analysis of Ergocalciferol, Cholecalciferol on Primesep SB by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method For Analysis  of  Vitamin D2 and D3 on Primesep SB Column_1401
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ergocalciferol, Cholecalciferol

High Performance Liquid Chromatography (HPLC) Method for Analysis of Ergocalciferol, Cholecalciferol


Ergocalciferol (Vitamin D2) and Cholecalciferol (Vitamin D3) are fat soluble vitamins that help your body absorb calcium and phosphorus. Your body can produce both when exposed to the sun, but you can also intake these essential vitamins through a daily multivitamin or a Vitamin D pill.
Vitamins D2 and D3 can be detected in the high UV regime. Using a Primesep SB reverse-phase column and a mobile phase consisting of water and acetonitrile with an acetic acid buffer, Vitamins D2 and D3 can be retained and separated. This analysis method can be UV detected at 265 nm.

ColumnPrimesep SB, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 80/20%
BufferAcetic Acid – 0.1%
Flow Rate1.0 ml/min
DetectionUV 265 nm
Class of Compounds
Vitamin
Analyzing CompoundsErgocalciferol (Vitamin D2),  Cholecalciferol (Vitamin D3)

Application Column

Primesep SB

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Cholecalciferol
Ergocalciferol

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.