HPLC Method For Analysis Of  Tetracycline on Primesep 100 Column

HPLC Method for Tetracycline hydrochloride, Tetracycline on Primesep 100 by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Tetracycline

Tetracycline is a broad spectrum antibiotic, that is, it is active against many different types of bacteria. It is effective against Chlamydia psittaci, Neisseria gonorrhoeae, Haemophilus influenzae, Streptococcus pneumoniae, Mycoplasma pneumoniae, Chlamydia trachomatis, and others. Tetracycline prevents the growth of bacteria by preventing the bacteria from manufacturing proteins they need to survive.

Tetracycline can be retained on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 200 nm.

Condition
Column Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 50/50%
Buffer H2SO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV, 200 nm

 

Description
Class of Compounds
 Drug, Antibiotics
Analyzing Compounds Tetracycline hydrochloride, Tetracycline

 

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Tetracycline
Tetracycline hydrochloride
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.