Tetracycline hydrochloride

Tetracycline hydrochloride structural formula

CAS Number64-75-5
Molecular FormulaC22H25ClN2O8
Molecular Weight480.900
InChI KeyXMEVHPAGJVLHIG-FMZCEJRJSA-N
LogP-1.17
Synonyms
  • Tetracycline hydrochloride
  • (4S,4aS,5aS,6S,12aS)-4-(Dimethylamino)-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-1,4,4a,5,5a,6,11,12a-octahydrotetracene-2-carboxamide--hydrogen chloride (1/1)
  • 2-Naphthacenecarboxamide, 4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-, (4S,4aS,5aS,6S,12aS)-, hydrochloride (1:1)
  • 64-75-5
  • 2-Naphthacenecarboxamide, 4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-, monohydrochloride, (4S,4aS,5aS,6S,12aS)-
  • (-)-Tetracycline hydrochloride
  • 2-Naphthacenecarboxamide, 4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-, hydrochloride (1:1), (4S,4aS,5aS,6S,12aS)-
  • 2-Naphthacenecarboxamide, 4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-, monohydrochloride
  • 2-Naphthacenecarboxamide, 4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-, monohydrochloride, [4S-(4?,4a?,5a?,6?,12a?)]-
  • 4-(Dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-2-naphthalenecarboxamide hydrochloride
  • Achromycin hydrochloride
  • Ala-Tet
  • Ambracyn
  • Bristaciclina
  • Bristacycline
  • Cancycline-250
  • Cefracycline
  • Criseociclina
  • Cyclopar
  • Diocyclin
  • Dumocyclin
  • Helvecyclin
  • Hostacyclin
  • Hostacycline
  • Medamycin
  • Neocycline B
  • Panmycin hydrochloride
  • Partrex
  • Polycycline hydrochloride
  • Polyotic ointment
  • Purocyclina
  • Quadracyclin
  • Remicyclin
  • Riocyclin
  • Robitet
  • Ro-Cycline
  • Sanclomycine
  • Steclin
  • Subamycin
  • Supramycin
  • Sustamycin
  • Tefilin
  • Tetrabakat
  • Tetrabid
  • Tetrablet
  • Tetrabon
  • Tetracaps
  • Tetrachel
  • tetraciclina, clorhidrato
  • Tetracompren
  • Tetracycline chlorohydrate
  • TETRACYCLINE HCL
  • Tetracycline, chlorhydrate
  • Tetracyclinhydrochlorid
  • Tetracyklin
  • Tetracyn
  • Tetrakap
  • Tetralution
  • Tetramavan
  • TetraSURE
  • Tetrosol
  • Topicycline
  • Totomycin
  • Triphacyclin
  • Vetquamycin 324
  • T-250 capsules
  • ALA TET
  • Artomycin
  • Cefracycline tablets
  • Diacycine
  • EINECS 200-593-8
  • Mephacyclin
  • NCI-C55561
  • Neocyclin B
  • Piracaps
  • Quadracycline
  • Quatrex
  • Ricycline
  • Stilciclina
  • Tetra-D
  • Tetracycline chloride
  • Tetracyn hydrochloride
  • Tetra-wedel
  • Vetquamycin-324
  • (-)-Tetrahcycline hydrochloride
  • Tetramed
  • Chlorhydrate de tetracycline
  • Chlorowodorku tetracykliny
  • Tetraciclina cloridrato
  • UNII-P6R62377KV
  • Sentry av ornacycline
  • 102030-19-3
  • 8017-77-4
  • 8026-72-0
  • 90438-83-8

Applications:

Uv-Vis Spectrum of Tetracycline

January 23, 2026
Access the UV-Vis Spectrum SIELC Library
UV-Vis Spectrum of Tetracycline hydrochloride, Tetracycline.

If you are looking for optimized HPLC method to analyze Tetracycline hydrochloride, Tetracycline check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

 

 

Application Analytes:
Tetracycline
Tetracycline hydrochloride
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method For Analysis Of  Tetracycline on Primesep 100 Column

August 9, 2021

HPLC Method for Tetracycline hydrochloride, Tetracycline on Primesep 100 by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Tetracycline

Tetracycline is a broad spectrum antibiotic, that is, it is active against many different types of bacteria. It is effective against Chlamydia psittaci, Neisseria gonorrhoeae, Haemophilus influenzae, Streptococcus pneumoniae, Mycoplasma pneumoniae, Chlamydia trachomatis, and others. Tetracycline prevents the growth of bacteria by preventing the bacteria from manufacturing proteins they need to survive.

Tetracycline can be retained on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 200 nm.

Condition
Column Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 50/50%
Buffer H2SO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV, 200 nm

 

Description
Class of Compounds
 Drug, Antibiotics
Analyzing Compounds Tetracycline hydrochloride, Tetracycline

 

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Tetracycline
Tetracycline hydrochloride
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Tetracycline hydrochloride on Newcrom R1 HPLC column

February 16, 2018
Separation of Tetracycline hydrochloride on Newcrom C18 HPLC column

Tetracycline hydrochloride can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Tetracycline hydrochloride
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.