Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Tetracycline
Tetracycline is a broad spectrum antibiotic, that is, it is active against many different types of bacteria. It is effective against Chlamydia psittaci, Neisseria gonorrhoeae, Haemophilus influenzae, Streptococcus pneumoniae, Mycoplasma pneumoniae, Chlamydia trachomatis, and others. Tetracycline prevents the growth of bacteria by preventing the bacteria from manufacturing proteins they need to survive. Tetracycline can be retained on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 200 nm.
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds |
Drug, Antibiotics |
| Analyzing Compounds | Tetracycline |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsTetracycline hydrochloride
