HPLC Method for Analysis of Semaglutide on Chromni Column

HPLC Method for Analysis of Semaglutide on Chromni Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Semaglutide.

Semaglutide is a GLP-1 receptor agonist with the chemical formula C187H291N45O59. It is used for managing type 2 diabetes and obesity. It mimics the effects of the natural hormone GLP-1, promoting insulin secretion, reducing glucagon levels, slowing gastric emptying, and suppressing appetite. These mechanisms help control blood sugar levels and support weight loss.. You can find detailed UV spectra of Semaglutide and information about its various lambda maxima by visiting the following link.

Semaglutide can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.

Condition

ColumnChromni, 4.6 x 150 mm, 3 µm, 100 A, dual ended
Mobile PhaseGradient MeCN/H2O
BufferH2SO4 – 0.2%
Flow Rate1.0 mL/min
DetectionUV 280 nm
Limit Of Detection*103.1 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsPeptide
Analyzing CompoundsSemaglutide

Application Column

Chromni

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Semaglutide
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.