| CAS Number | 910463-68-2 |
|---|---|
| Molecular Formula | C187H291N45O59 |
| Molecular Weight | 4114 |
| InChI Key | DLSWIYLPEUIQAV-CCUURXOWSA-N |
| LogP | -5.8 |
| Synonyms |
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Applications:
UV-Vis Spectrum of Semaglutide
March 5, 2025
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC-MS Method for Analysis of Semaglutide on Solid-Core Primesep SB Column
February 26, 2025
HPLC Method for Semaglutide on Solid-Core Primesep SB by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Semaglutide
Semaglutide is a GLP-1 receptor agonist used for managing type 2 diabetes and obesity. It mimics the effects of the natural hormone GLP-1, promoting insulin secretion, reducing glucagon levels, slowing gastric emptying, and suppressing appetite. These mechanisms help control blood sugar levels and support weight loss.
Semaglutide can be retained and analyzed using the Solid-Core Primesep SB stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and Ammonium formate. Detection was performed using UV absorption at 280 nm. Mass spectrometric detection was carried out in ESI-SIM mode, monitoring the ions [M+3H]³⁺ at m/z 1372 and [M+4H]⁴⁺ at m/z 1029
| Column | Solid-Core Primesep SB, 3.2 x 100 mm, 2.7 µm, 90 A |
| Mobile Phase | Gradient MeCN 40-80%, 10 min |
| Buffer | Ammonium Formate pH 3.0 – 40 mM |
| Flow Rate | 0.5 ml/min |
| Detection | UV 280 nm, ESI-SIM: [M+3H]3⁺ -1372, [M+4H]4⁺ -1029 |
| LOD | 3 ppb |
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
| Class of Compounds | GLP-1 Receptor Agonist |
| Analyzing Compounds | Semaglutide |
Application Column
Solid-Core Primesep SB
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 2.7 µm
Pore Size: 90 A
UV Detection
HPLC Method for Analysis of Semaglutide on Solid-Core Primesep SB Column
February 24, 2025
HPLC Method for Semaglutide on Solid-Core Primesep SB by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Semaglutide
Semaglutide is a GLP-1 receptor agonist used for managing type 2 diabetes and obesity. It mimics the effects of the natural hormone GLP-1, promoting insulin secretion, reducing glucagon levels, slowing gastric emptying, and suppressing appetite. These mechanisms help control blood sugar levels and support weight loss.
Semaglutide can be retained and analyzed using the Solid-Core Primesep SB stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and TFA. Detection is performed using UV at 280 nm.
| Column | Solid-Core Primesep SB, 3.2 x 100 mm, 2.7 µm, 90 A |
| Mobile Phase | Gradient MeCN 30-70%, 10 min |
| Buffer | TFA – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 280 nm |
| LOD | 59.4 ppb |
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
| Class of Compounds | GLP-1 Receptor Agonist |
| Analyzing Compounds | Semaglutide |
Application Column
Solid-Core Primesep SB
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 2.7 µm
Pore Size: 90 A
