HPLC Method for Semaglutide on Solid-Core Primesep SB by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Semaglutide
Semaglutide is a GLP-1 receptor agonist used for managing type 2 diabetes and obesity. It mimics the effects of the natural hormone GLP-1, promoting insulin secretion, reducing glucagon levels, slowing gastric emptying, and suppressing appetite. These mechanisms help control blood sugar levels and support weight loss.
Semaglutide can be retained and analyzed using the Solid-Core Primesep SB stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and Ammonium formate. Detection was performed using UV absorption at 280 nm. Mass spectrometric detection was carried out in ESI-SIM mode, monitoring the ions [M+3H]³⁺ at m/z 1372 and [M+4H]⁴⁺ at m/z 1029
| Column | Solid-Core Primesep SB, 3.2 x 100 mm, 2.7 µm, 90 A |
| Mobile Phase | Gradient MeCN 40-80%, 10 min |
| Buffer | Ammonium Formate pH 3.0 – 40 mM |
| Flow Rate | 0.5 ml/min |
| Detection | UV 280 nm, ESI-SIM: [M+3H]3⁺ -1372, [M+4H]4⁺ -1029 |
| LOD | 3 ppb |
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
| Class of Compounds | GLP-1 Receptor Agonist |
| Analyzing Compounds | Semaglutide |
Application Column
Solid-Core Primesep SB
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 2.7 µm
Pore Size: 90 A
UV Detection
