HPLC Method for Analysis of Semaglutide on Solid-Core Primesep SB Column

HPLC Method for Semaglutide on Solid-Core Primesep SB by SIELC Technologies

HPLC Method for Analysis of Semaglutide on Solid-Core Primesep SB Column by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Semaglutide

Semaglutide is a GLP-1 receptor agonist used for managing type 2 diabetes and obesity. It mimics the effects of the natural hormone GLP-1, promoting insulin secretion, reducing glucagon levels, slowing gastric emptying, and suppressing appetite. These mechanisms help control blood sugar levels and support weight loss.

Semaglutide can be retained and analyzed using the Solid-Core Primesep SB stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and TFA. Detection is performed using UV at 280 nm.

ColumnSolid-Core Primesep SB, 3.2 x 100 mm, 2.7 µm, 90 A
Mobile PhaseGradient MeCN 30-70%, 10 min
BufferTFA – 0.1%
Flow Rate0.5 ml/min
DetectionUV 280 nm
LOD59.4 ppb

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsGLP-1 Receptor Agonist
Analyzing CompoundsSemaglutide

Application Column

Solid-Core Primesep SB

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 2.7 µm
Pore Size: 90 A

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Application Analytes:
Semaglutide
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.