HPLC Method for Analysis of Pentosidine

 

HPLC Method for Pentosidine on Obelisc R by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Pentosidine

Pentosidine is a advanced glycation end-product (AGE) with C17H26N6O4 • CF3COOH chemical formula.  Since it is a fluorescent biomarker, it is often used as an indicator  in studies of aging and chronic diseases including diabetes and uremia.

Pentosidine can be retained and analyzed using the Obelisc R stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium acetate buffer. Detection is performed using UV.

ColumnObelisc R, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 20%
BufferAmmonium acetate
Flow Rate1.0 ml/min
DetectionUV
Class of CompoundsAdvanced Glycation End-Product
Analyzing CompoundsPentosidine

Application Column

Obelisc R

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Pentosidine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.