HPLC Method for Analysis of Mannitol on Primesep S2 Column

HPLC Method for Mannitol on Primesep S2 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Mannitol

Mannitol is an organic compound with the molecular formula C6H14O6.

Properties:
Appearance: Typically a white, odorless, crystalline powder or granules.

Molecular weight: ~182.17 g/mol

Solubility: Soluble in water.

Uses: A versatile medication with various uses in healthcare, primarily as an osmotic diuretic. 

Mannitol can be retained and analyzed using the Primesep S2 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and formic acid. Detection is performed using ELSD.

ColumnPrimesep S2, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 85%
BufferFormic Acid – 0.2%
Flow Rate1.0 ml/min
DetectionELSD
LOD4.380 ppm

Class of CompoundsSugar Alcohol
Analyzing CompoundsMannitol

Application Column

Primesep S2

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Mannitol
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.