HPLC Method for Arginine, Carnitine on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Arginine, Carnitine.
L-carnitine is a quaternary ammonium cation with the chemical formula C7H15NO3. It is key to the metabolic processes of most living species as is vital in fat metabolism, mitochondrial protection, and cellular energy. It works to transport long-chain fatty acids onto the mitochondria. It is produced naturally in the body, but is also often consumed as a dietary supplement. You can find detailed UV spectra of L-carnitine and information about its various lambda maxima by visiting the following link.
L-Arginine is an amino acid with the chemical formula C9H11NO3. It is a precursor for nitric acid, making it crucial for blood flow and general cardiovascular health. It can help lower blood pressure, help with chest pain, and improve symptoms of Peripheral arterial disease (PAD). You can find detailed UV spectra of L-Arginine and information about its various lambda maxima by visiting the following link.
Arginine, Carnitine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | Gradient H2SO4 from 0.1 to 0.5%, 10 min |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 205 nm |
| Class of Compounds |
Amino acid |
| Analyzing Compounds | Arginine, Carnitine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Carnitine
