| CAS Number | 406-76-8 |
|---|---|
| Molecular Formula | C7H15NO3 |
| Molecular Weight | 161.20 |
| InChI Key | PHIQHXFUZVPYII-UHFFFAOYSA-N |
| LogP | -0.2 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of L-Carnitine
January 9, 2026
If you are looking for optimized HPLC method to analyze Carnitine check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method For Analysis Of L-Carnitine and Arginine on Primesep 100 Column
February 3, 2022
HPLC Method for Arginine, Carnitine on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Arginine, Carnitine.
L-carnitine is a quaternary ammonium cation with the chemical formula C7H15NO3. It is key to the metabolic processes of most living species as is vital in fat metabolism, mitochondrial protection, and cellular energy. It works to transport long-chain fatty acids onto the mitochondria. It is produced naturally in the body, but is also often consumed as a dietary supplement. You can find detailed UV spectra of L-carnitine and information about its various lambda maxima by visiting the following link.
L-Arginine is an amino acid with the chemical formula C9H11NO3. It is a precursor for nitric acid, making it crucial for blood flow and general cardiovascular health. It can help lower blood pressure, help with chest pain, and improve symptoms of Peripheral arterial disease (PAD). You can find detailed UV spectra of L-Arginine and information about its various lambda maxima by visiting the following link.
Arginine, Carnitine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | Gradient H2SO4 from 0.1 to 0.5%, 10 min |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 205 nm |
| Class of Compounds |
Amino acid |
| Analyzing Compounds | Arginine, Carnitine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Carnitine


