Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of L-Carnitine and Arginine
L-carnitine is a quaternary ammonium cation (also known as a quat) that is key to the metabolic processes of most living species. Arginine is an alpha amino acid that is key to the biosynthesis of proteins.
These two amine-based compounds can be detected in the low UV regime. Using a Primesep 100 mixed-mode column and a gradient mobile phase consisting of water and acetonitrile (MeCN) with a gradient of sulfuric acid (H2SO4) as a buffer, L-carnitine and Arginine can be retained, separated, and analyzed. This analysis method can be UV detected at 205 nm.
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | Gradient H2SO4 from 0.1 to 0.5%, 10 min |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 205 nm |
| Class of Compounds |
Amino acid |
| Analyzing Compounds | Carnitine, Arginine |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsCarnitine
