HPLC Method for Analysis of Diclofenac and Carbamazepine on Primesep 100 Column on Alltesta™

HPLC Method for Diclofenac, Carbamazepine on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Diclofenac, Carbamazepine

Diclofenac is a nonsteroidal anti-inflammatory drug (NSAID) with the molecular formula C14H11Cl2NO2. It is a pain killer that also relieves symptoms of arthritis. It is only accessible through a prescription from a medical professional.

Carbamazepine is a anticonvulsant medication with the molecular formula  C15H12N2O. It is typically used to treat epilepsy, bipolar disorder, and nerve pain. It works through calming overactive nerves through binding voltage-dependent sodium channels.

Diclofenac, Carbamazepine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 35%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 275 nm

Class of CompoundsMedication
Analyzing CompoundsDiclofenac, Carbamazepine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Carbamazepine
Diclofenac
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.