HPLC Method for Analysis of Desmosine

HPLC Method for Desmosine on Obelisc R by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Desmosine 

Desmosine is pyridinium-based amino acid derivative with C24H40N5O8+ molecular formula. It is found exclusively in elastin. Elevated levels of desmosine in urine can be a symptom of Chronic Obstructive Pukmonary Disease (COPF), so it can be used as a biomarker when monitoring progression and aiding in a potential diagnosis.

Desmosine can be retained and analyzed using the Obelisc R stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium acetate buffer. Detection is performed using UV.

ColumnObelisc R, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 10%
BufferAmAcetate – 30 mM pH 5.0
Flow Rate1.0 ml/min
DetectionUV 270nm
Class of CompoundsAmino Acid
Analyzing CompoundsDesmosine

Application Column

Obelisc R

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Desmosine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.