Desmosine

CAS Number11003-57-9
Molecular FormulaC24H40N5O8
Molecular Weight526.29
InChI KeyVEVRNHHLCPGNDU-MUGJNUQGSA-O
LogP-7.26
Synonyms

    Applications:

    HPLC Determination of Desmosine on Primesep C Column

    January 6, 2021


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    Desmosine and isodesmosine are unique amino acids found in elastin, a protein in connective tissue such as elastic arteries, lungs, and skin.

    Desmosine and isodesmosine can be used as specific biological markers for the degradation of mature cross-linked elastin.

    Desmosine can be detected at 270nm using Primesep C mixed-mode reversed-phase cation-exchange column and a mobile phase consisting of acetonitrile (ACN), and water with Ammonium acetate buffer.

    Condition 1

    Column Primesep C, 4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN – 10%
    Buffer Ammonium Acetate pH 5.0 – 20 mM
    Flow Rate 1.0 mL/min
    Detection UV 270 nm

    Condition 2

    Column Primesep C, 4.6×150 mm, 5 µm, 100A,
    Guard Newcrom R1 4.6×50 mm, 5µm, 100A,
    Mobile Phase MeCN – 10%
    Buffer Ammonium Acetate pH 5.0 – 20 mM
    Flow Rate 1.0 mL/min
    Detection UV 270 nm

    Description

    Class of Compounds
    Aminoacid, Poly-amino acid
    Analyzing Compounds Desmosine

     

    Application Column

    Primesep C

    Column Diameter: 4.6 mm
    Column Length: 150 mm
    Particle Size: 5 µm
    Pore Size: 100 A

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    Application Analytes:
    Desmosine

    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Method for Analysis of Desmosine

    March 16, 2018

    Application Column

    Obelisc R

    SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

    Select options
    Application Analytes:
    Desmosine
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.