HPLC Determination of Desmosine on Primesep C Column

HPLC.cloud View on hplc.cloud Desmosine and isodesmosine are unique amino acids found in elastin, a protein in connective tissue such as elastic arteries, lungs, and skin. Desmosine and isodesmosine can be used as specific biological markers for the degradation of mature cross-linked elastin. Desmosine can be detected at 270nm using Primesep C mixed-mode reversed-phase cation-exchange column and a mobile phase consisting of acetonitrile (ACN), and water with Ammonium acetate buffer.

Condition 1

Column Primesep C, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 10%
Buffer Ammonium Acetate pH 5.0 - 20 mM
Flow Rate 1.0 mL/min
Detection UV 270 nm

Condition 2

Column Primesep C, 4.6x150 mm, 5 µm, 100A,
Guard Newcrom R1 4.6x50 mm, 5µm, 100A,
Mobile Phase MeCN - 10%
Buffer Ammonium Acetate pH 5.0 - 20 mM
Flow Rate 1.0 mL/min
Detection UV 270 nm

Description

Class of Compounds Aminoacid, Poly-amino acid
Analyzing Compounds Desmosine
 
Application Analytes:
Desmosine