HPLC Determination of Desmosine on Primesep C Column

HPLC Method for Desmosine on Primesep C by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Desmosine.

Desmosine and isodesmosine are unique amino acids found in elastin, a protein in connective tissue such as elastic arteries, lungs, and skin. Both can be used as specific biological markers for the degradation of mature cross-linked elastin.

Desmosine is pyridinium-based amino acid derivative with C24H40N5O8+ molecular formula. It is found exclusively in elastin. Elevated levels of desmosine in urine can be a symptom of Chronic Obstructive Pukmonary Disease (COPF), so it can be used as a biomarker when monitoring progression and aiding in a potential diagnosis.

Desmosine can be detected at 270nm using Primesep C mixed-mode reversed-phase cation-exchange column and a mobile phase consisting of acetonitrile (ACN), and water with Ammonium acetate buffer.

Condition 1
Column Primesep C, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 10%
Buffer Ammonium Acetate pH 5.0 – 20 mM
Flow Rate 1.0 mL/min
Detection UV 270 nm

Condition 2
Column Primesep C, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Guard Newcrom R1 4.6×50 mm, 5µm, 100A,
Mobile Phase MeCN – 10%
Buffer Ammonium Acetate pH 5.0 – 20 mM
Flow Rate 1.0 mL/min
Detection UV 270 nm

Description
Class of Compounds
 Aminoacid, Poly-amino acid
Analyzing Compounds Desmosine

 

Application Column

Primesep C

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Desmosine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.