Desmosine and isodesmosine are unique amino acids found in elastin, a protein in connective tissue such as elastic arteries, lungs, and skin.
Desmosine and isodesmosine can be used as specific biological markers for the degradation of mature cross-linked elastin.
Desmosine can be detected at 270nm using Primesep C mixed-mode reversed-phase cation-exchange column and a mobile phase consisting of acetonitrile (ACN), and water with Ammonium acetate buffer.
Column | Primesep C, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN – 10% |
Buffer | Ammonium Acetate pH 5.0 – 20 mM |
Flow Rate | 1.0 mL/min |
Detection | UV 270 nm |
Column | Primesep C, 4.6×150 mm, 5 µm, 100A, |
Guard | Newcrom R1 4.6×50 mm, 5µm, 100A, |
Mobile Phase | MeCN – 10% |
Buffer | Ammonium Acetate pH 5.0 – 20 mM |
Flow Rate | 1.0 mL/min |
Detection | UV 270 nm |
Class of Compounds |
Aminoacid, Poly-amino acid |
Analyzing Compounds | Desmosine |
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsPrimesep C
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options