HPLC Method for Analysis of Chlorpyrifos (Dursban) on Newcrom B Column on Alltesta™

HPLC Method for Chlorpyrifos-methyl (Dursban) on Newcrom B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Chlorpyrifos-methyl (Dursban) 

Chlorpyrifos, also known as Dursban, is a highly toxic organophosphate with the chemical formula C9H11Cl3NO3PS. It works through interrupting the electrochemical processes in nerves.  This leads to a build-up of acetylcholine, which leads to paralysis and eventual death.

Chlorpyrifos-methyl (Dursban) can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.

ColumnNewcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 70%
BufferAmmonium Formate
Flow Rate1.0 mL/min
DetectionUV 275 nm
Class of CompoundsPesticide
Analyzing CompoundsChlorpyrifos-methyl (Dursban)

Application Column

Newcrom B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Chlorpyrifos-methyl (Dursban)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.