HPLC Method for Brilliant Blue FCF (Blue 1), Allura Red (Red #40) on Newcrom BH by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Brilliant Blue FCF (Blue 1), Allura Red (Red #40).
Brilliant Blue FCF (Blue 1) is a synthetic molecule used as a food coloring agent. Allura Red (Red 40) is another food colorant. Both are present in Monster Energy Ultra Violet sugar free drink. Both are disodium salts and can be separated in HPLC by our Newcrom BH mixed-mode column which utilizes both hydrophobic and ion-exchange properties. The mobile phase is methanol (MeOH) and water with sulfuric acid (H2SO4) buffer making the method MS-compatible. Can also be UV detected at 210nm
You can find detailed UV spectra of Blue 1 and information about its various lambda maxima by visiting the following link.
You can find detailed UV spectra of Red 40 and information about its various lambda maxima by visiting the following link.
| Column | Newcrom BH, 4.6 x 50 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeOH/H2O – 75/25% |
| Buffer | H2SO4 – 0.5%% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Class of Compounds | Acid, Dyes |
| Analyzing Compounds | Brilliant Blue FCF (Blue 1), Allura Red (Red #40) |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Brilliant Blue FCF (Blue 1)
