HPLC Method for Analysis of Acetaminophen on Primesep 100 Column on Alltesta™

HPLC Method for Acetaminophen (Paracetamol) on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Acetaminophen (Paracetamol)

Acetaminophen, also known as Paracetamol, is a medication with the molecular formula C8H9NO2. It is an over-the-counter pain killer that also reduces fever. Unlike Ibuprofen and naproxen, it is not an anti-inflammatory drug.

Acetaminophen (Paracetamol) can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 10%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 275 nm

Class of CompoundsOrganic Compound
Analyzing CompoundsAcetaminophen (Paracetamol)

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Acetaminophen (Paracetamol)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.