HPLC Method for Analysis of 4-Amino-2-nitrophenol on Primesep 100 Column with Alltesta™

HPLC Method for 4-Amino-2-nitrophenol on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of 4-Amino-2-nitrophenol

4-Amino-2-nitrophenol is a hydrocarbon with the molecular formula C6H6N2O3. It is a red-purple powder that is typically used in as an oxidation base in hair dye. California considers this compound a carcinogen.

4-Amino-2-nitrophenol can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

ColumnPrimesep 100, 3.2 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 50%
BufferSulfuric Acid
Flow Rate1.0 ml/min
DetectionUV 275 nm

Class of CompoundsHydrocarbon
Analyzing Compounds4-Amino-2-nitrophenol

Application Column

Primesep 100

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
4-Amino-2-nitrophenol
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.