4-Amino-2-nitrophenol

4-Amino-2-nitrophenol structural formula

CAS Number119-34-6
Molecular FormulaC6H6N2O3
Molecular Weight154.125
InChI KeyWHODQVWERNSQEO-UHFFFAOYSA-N
LogP0.96
Synonyms
  • 4-Amino-2-nitrophenol
  • Phenol, 4-amino-2-nitro-
  • 119-34-6
  • 4-13-00-01284
  • C.I. Oxidation Base 25
  • 1-Amino-3-nitro-4-hydroxybenzene
  • p-Amino-o-nitrophenol
  • BRN 1368435
  • CI Oxidation Base 25
  • EINECS 204-316-1
  • Fourrine 57
  • Fourrine Brown PR
  • Fourrine Brown Propyl
  • 4-Hydroxy-3-nitroaniline
  • NCI-C03963
  • o-Nitro-p-aminophenol
  • 2-Nitro-4-aminophenol
  • 3-Nitro-4-hydroxyaniline
  • NSC 56809
  • ortho-Nitro-para-aminophenol
  • Oxidation Base 25
  • 4-Amino-2-nitrofenol
  • UNII-N3499RCQ6B

Applications:

 HPLC Method for Analysis of  4-Amino-2-nitrophenol on Primesep 100  Column

May 15, 2025

HPLC Method for 4-Amino-2-nitrophenol on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of 4-Amino-2-nitrophenol

4-Amino-2-nitrophenol is an organic compound with the molecular formula C₆H₆N₂O₃.

Properties:
Appearance: Typically a yellow to orange crystalline solid

Molecular weight: ~154.12 g/mol

Solubility: Soluble in water and polar organic solvents

Uses: Intermediate in the manufacture of dyes, pigments, and pharmaceuticals

4-Amino-2-nitrophenol can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid. Detection is performed using UV at 275 nm.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 50%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 275 nm
LOD93 ppb

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsAromatic amine
Analyzing Compounds4-Amino-2-nitrophenol

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
4-Amino-2-nitrophenol
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of 4-Amino-2-nitrophenol on Newcrom R1 HPLC column

February 16, 2018
Separation of 4-Amino-2-nitrophenol on Newcrom C18 HPLC column

4-Amino-2-nitrophenol can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
4-Amino-2-nitrophenol
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.