HPLC Method for 4-Amino-2-nitrophenol on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of 4-Amino-2-nitrophenol
4-Amino-2-nitrophenol is an organic compound with the molecular formula C₆H₆N₂O₃.
Properties:
Appearance: Typically a yellow to orange crystalline solid
Molecular weight: ~154.12 g/mol
Solubility: Soluble in water and polar organic solvents
Uses: Intermediate in the manufacture of dyes, pigments, and pharmaceuticals
4-Amino-2-nitrophenol can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid. Detection is performed using UV at 275 nm.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN – 50% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm |
| LOD | 93 ppb |
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
| Class of Compounds | Aromatic amine |
| Analyzing Compounds | 4-Amino-2-nitrophenol |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
